KMID : 0379520160320030225
|
|
Çѱ¹µ¶¼ºÇÐȸÁö 2016 Volume.32 No. 3 p.225 ~ p.230
|
|
Cytotoxicity Evaluation of Essential Oil and its Component from Zingiber officinale Roscoe
|
|
Lee Yong-Kyu
|
|
Abstract
|
|
|
Zingiber officinale Roscoe has been widely used as a folk medicine to treat various diseases, including cancer. This study aims to re-examine the therapeutic potential of co-administration of natural products and cancer chemotherapeutics. Candidate material for this project, ¥á-zingiberene, was extracted from Zingiber officinale Roscoe, and ¥á-zingiberene makes up 35.02 ¡¾ 0.30% of its total essential oil. ¥á-Zingiberene showed low IC50 values, 60.6 ¡¾ 3.6, 46.2 ¡¾ 0.6, 172.0 ¡¾ 6.6, 80.3 ¡¾ 6.6 (¥ìg/mL) in HeLa, SiHa, MCF-7 and HL-60 cells each. These values are a little bit higher than IC50 values of general essential oil in those cells. The treatment of ¥á-zingiberene produced nucleosomal DNA fragmentation in SiHa cells, and the percentage of sub-diploid cells increased in a concentration-dependent manner in SiHa cells, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of ¥á-zingiberene, which mediates cell death. These results suggest that the apoptotic effect of ¥á-zingiberene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c into cytoplasm. It is considered that anti-proliferative effect of ¥á-zingiberene is a result of apoptotic effects, and ¥á-zingiberene is worth furthermore study to develop it as cancer chemotherapeutics.
|
|
KEYWORD
|
|
Cytotoxic activity, ¥á-Zingiberene, Anti-proliferation, General essential oil, Cervix cancer cells
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|